Analysis of full-length gene sequence of rabies vaccine virus aG strain.
- Author:
Jia LI
1
;
Shou-Chun CAO
;
Lei-Tai SHI
;
Xiao-Hong WU
;
Jing-Hua LIU
;
Yun-Peng WANG
;
Jian-Rong TANG
;
Yong-Xin YU
;
Guan-Mu DONG
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050,China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Antigens, Viral;
genetics;
immunology;
Base Sequence;
China;
Genome, Viral;
genetics;
Genotype;
Humans;
Molecular Sequence Data;
Phylogeny;
Rabies;
immunology;
prevention & control;
virology;
Rabies Vaccines;
immunology;
Rabies virus;
genetics;
immunology;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Analysis, DNA;
Sequence Homology, Amino Acid;
Species Specificity
- From:
Chinese Journal of Virology
2013;29(4):404-409
- CountryChina
- Language:Chinese
-
Abstract:
To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full-length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5' leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4. 0 software. aG strain was 11 925nt(GenBank accession number: JN234411) in length and belonged to the genotype I . The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus. the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.
