The use of dental papillae cells and millipore filter for bioengineered dentin
10.3760/cma.j.issn.1002-0098.2010.11.011
- VernacularTitle:大鼠牙乳头细胞复合微孔滤膜形成牙本质样结构的实验研究
- Author:
Wei-Jia NI
1
;
Yu-Cheng LI
;
Jia-Yuan WU
;
Long-Xing NI
Author Information
1. 第四军医大学口腔医学院
- Keywords:
Dental papilla;
Micropore filters;
Cell differentiation;
Dentin regeneration
- From:
Chinese Journal of Stomatology
2010;45(11):678-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β1 (TGF-β1). Methods The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass.The cell mass was seeded on the millipore filter combined with TGF-β1. The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed. Results A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein(DSP) and dentin matrix protein-1 ( DMP-1 ) were positive in these cells.After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter. Conclusions The millipore filter combined with TGF-β1 could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.
