In vivo short hairpin RNA interference of vascular endothelial growth factor on drug-resistant tongue cancer cells
10.3760/cma.j.issn.1002-0098.2011.01.007
- VernacularTitle:短发夹RNA干扰血管内皮生长因子对舌癌耐药细胞移植瘤的影响
- Author:
Xiao-Lin NONG
1
;
Hao LI
;
Yong XIA
;
Yan LI
;
Jia-Quan LI
;
Yi-Ping YANG
Author Information
1. 广西医科大学口腔医学院
- Keywords:
RNA interference;
Vascular endothelial growth factor;
Drug resistance,neoplasm;
Tongue neoplasm
- From:
Chinese Journal of Stomatology
2011;46(1):15-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the in vivo interference effects of vascular endothelial growth factor (VEGF) short hairpin RNA (shRNA) on xenografts of drug-resistant tongue cancer cells. Methods Drugresistant tongue caner cells Tca/Cisplatin(DDP) were injected subcutaneously into nude mice to establish xenograft models, which were randomly divided into non-transfected group, mock control group, control group transfected with scrambled sequence plasmid, interference group transfected with VEGF-shRNA expression plasmid. Liposome-mediated plasmid transfection was done in the latter three groups every three days. Xenografts were observed and tumor growth curve was measured. After the 10th transfection, tumors were anatomized and weigh. Microvessel density was detected by immunohistochemical staining. In situhybridization assay was used to test VEGF mRNA, and immunohistochemistry to test VEGF, P-glycoprotein ( P-gp), B cell lymphoma/leukemia-2 ( bcl-2 ) and extracellular signal-regultaed kinase 2 (ERK-2) protein. Results Tumor growth in VEGF-shRNA interference group was significantly slow. Tumor weight was (0.4781 ±0.0860) g, microvessel density (7.35 ± 1.31)/view, VEGF mRNA(0.0767 ±0.0234), VEGF protein (0.1301 ± 0.0433 ), P-gp (0.1517 ± 0.0184 ), bcl-2 ( 0.1218 ± 0.0251 ) and ERK-2 protein (0.1178 ±0.0291 ) in VEGF-shRNA interference group; all of them were less than those in the other three groups( P ＜ 0.05 ). Conclusions Inhibition targeting VEGF may become a potential therapy for drugresistant tongue cancer.
