Mechanism of advanced glycation end-products in the inducement of apoptosis of human gingival fibroblast and related effect of puerarin in the process
10.3760/cma.j.issn.1002-0098.2011.01.011
- VernacularTitle:糖基化终产物促进人牙龈成纤维细胞凋亡机制及葛根素拮抗作用初探
- Author:
Hui-Xia XU
1
;
Yun FU
;
Hua-Jing LI
Author Information
1. 中山大学光华口腔医学院(口腔医院)
- Keywords:
Fibroblasts;
Glycosylation end products,advanced;
Puerarin;
Apoptosis
- From:
Chinese Journal of Stomatology
2011;46(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of synthesized advanced glycation end-products(AGE)on reactive oxygen species formation and apoptosis of the cultured human gingival fibroblast and investigate the potential mechanisms of AGE in the modification of periodontal impairment. Methods AGE products with different concentrations[0, 50, 150 mg/L AGE-human serum albumin (AGE-HSA)] were incubated with human gingival fibroblast for 48 h, respestively. Flow cytometry was used to detect intracellular reactive oxygen species and cell apoptosis. The culture media with 50 mg/L AGE-HSA was exposed to 0.24 mmol/L puerarin for 48 h and then cell apoptosis was measured. Results The values of cellular apoptotic rate in 0,50, 150 mg/L AGE-HSA groups were ( 1.60 ± 0.30 ) %, ( 29.43 ± 1.45 ) %, ( 49. 20 ± 4. 43 ) %,respectively. The differences between each AGE-HSA group and control were statistically significant ( P ＜0.05). AGE-HSA increased cell apoptosis in a dose-dependent manner ( 150 mg/L ＞ 50 mg/L ＞0 mg/L,P ＜0.05 ). The cellular fluorescence intensity value was elevated as the concentration of AGE-HSA increased ( P ＜ 0.05 ). After incubation of human gingival fibroblast with AGE-HSA for 48 h, there was a significant decrease in apoptotic rate in puerarin group [ ( 6. 37 ± 3.02 ) % ], compared with the control [ ( 29. 43 ±1.45 ) %, P ＜ 0.05 ]. Conclusions AGE can stimulate apoptosis of human gingival fibroblast, which may be mediated by oxidative stress. Puerarin may protect periodontal tissues by inhibiting the apoptosis.
