Correlation between the CD68 proportion of peripheral blood mononuclear cell and macrophage infiltration during acute rejection of rat oral mucosal xenotransplantation
10.3760/cma.j.issn.1002-0098.2011.01.012
- VernacularTitle:大鼠口腔黏膜急性排斥反应外周血CD68水平与巨噬细胞浸润的相关性研究
- Author:
Wen-Xin ZUO
1
;
Hong WANG
;
Xiao-Yu LI
;
Xiao-An TAO
;
Bin CHENG
Author Information
1. 暨南大学第二临床医学院(深圳市人民医院)
- Keywords:
Mouth mucosa;
Graft rejection;
Mononcytes;
Macrophages
- From:
Chinese Journal of Stomatology
2011;46(1):35-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the dynamic process of peripheral blood CD68 mononuclear cells proportion and macrophages inflitration and possible correlation between them during acute rejection of rat oral mucosal xenotransplantation. Methods Thirty-six female wistar rats were divided into three groups randomly, including xenotransplantation group( n = 15 ), trauma control group( n = 12) and normal control group ( n = 9). The rat oral mucosa xenotransplantation model was established. The flow cytometry was used to evaluate the peripheral blood CD68 mononuclear cell and immunohistochemical assay performed to detect the macrophages infiltration one week (W1), two weeks (W2)and four weeks (W4) after xenotransplantation. Results The peripheral blood CD68 mononuclear cells percentage of each xenotransplantation group presented a rise and fall tendency at the three time points, and the peak value appeared at W4(43. 1% ), and the nadir at W2( 10.4% ). The macrophage counts achieved peak value in xenotransplantation group at W1 [ 580.0 (195.5) cell/high power field ], and then reduced with time. Conclusions The mononuclear cells and macrophage were capable of recognizing the xenograft and directly participated the acute rejection of rat oral mucosal xenotransplantation. The peripheral blood mononuclear cells percentage could reflect macrophage infiltrating condition at the early stage of the acute rejection.
