Scanning electron microscopy observation of the growth of osteoblasts on Ti-24Nb-4Zr-8Sn modified by micro-arc oxidation and alkali-heat treatment and implant-bone interface.
- Author:
Xue HAN
1
;
Hong-Chen LIU
;
Dong-Sheng WANG
;
Shu-Jun LI
;
Rui YANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkalies; Alloys; chemistry; Animals; Cells, Cultured; Dental Alloys; Dogs; Hot Temperature; Mice; Microscopy, Electron, Scanning; Osseointegration; Osteoblasts; cytology; Oxidation-Reduction; Prostheses and Implants; Prosthesis Implantation; Surface Properties; Tibia; surgery
- From: Chinese Journal of Stomatology 2011;46(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the efficacy of micro-arc oxidation and alkali-heat treatment (MAH) on Ti-24Nb-4Zr-8Sn (Ti2448).
METHODSDisks (diameter of 14.5 mm, thickness of 1 mm) and cylinders (diameter of 3 mm, height of 10 mm) were fabricated from Ti2448 alloy. Samples were divided into three groups: polished (Ti2448), micro-arc oxidation(MAO-Ti2448), micro-arc oxidation and alkali-heat treatment (MAH-Ti2448). MC3T3-E1 osteoblastic cells were cultured on the disks and cell morphology was observed with scanning electron microscopy (SEM) aftre 3 days. The cylinder samples were implanted in the tibia of dogs and implant-bone interface was observed with SEM after 3 months.
RESULTSA rough and porous structure was shown in both MAO and MAH group. The MC3T3-E1 cells on the MAH-Ti2448 discs spread fully in intimate contact with the underlying coarse surface through active cytoskeletal extentions. Osseointegration was formed in the implant-bone interface in MAH samples.
CONCLUSIONSMAH treatment can provide a more advantageous Ti2448 surface to osteoblastic cells than MAO treatment does, and the former can improve the implant-bone integration.
