Anti-sense miRNA-21 oligonucleotide inhibits Tb 3. 1 human tongue squamous cell carcinoma growth in vitro
10.3760/cma.j.issn.1002-0098.2011.02.004
- VernacularTitle:反义微小RNA-21寡核苷酸抑制Tb3.1人舌鳞状细胞癌增殖的体外研究
- Author:
Ying-Jie TAO
1
;
Yu REN
;
Jia-Bin DONG
;
Lun ZHANG
;
Jun-Ping CHENG
;
Xuan ZHOU
Author Information
1. 天津医科大学附属肿瘤医院
- Keywords:
Tongue neoplasms;
Carcinoma,squamous cell;
Genes regulator
- From:
Chinese Journal of Stomatology
2011;46(2):79-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of micro RNA-21 (miRNA-21) knocking on the Tb3.1 human tongue squamous cell carcinoma growth. Methods Anti-sense miRNA-21 oligonucleotide was delivered with oligofectamine to suppress Tb 3. 1 tongue cancer cell growth in vitro. Real-time polymerase chain reaction (PCR) was conducted to detect the miRNA-21 expression after transfection. Methyl thiazolyl tetrazolium(MTT) assay was used to determine Tb 3. 1 cell survival rate. Apoptosis were examined by flowcytometry. Matrigel matrix and transwell assay were used to determine Tb 3.1 cell colony formation and migration ability. Antigen KI-67 (Ki67), B cell lymphoma (Bcl-2), phosphatase and tensin homolog (PTEN), matrirx metalloproteinase 2(MMP-2, MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) protein expression in Tb 3. 1 cell were measured by Western blotting. Results miRNA-21 expression was decreased in miRNA-21 antisense oligonucleotide (ASODN) group. The survival rate of Tb 3. 1 cells with AS-miRNA-21 transfection was significantly suppressed (F=27.02, P = 0.00) and early phase apoptosis(F =26. 641 ,P = 0. 001) induced in Tb 3.1 cell. Ki67, Bcl-2, MMP-2 and MMP-9 protein weredown regulated while PTEN and TIMP-1 protein expression was increased. Conclusions Blocking miRNA-21 expression in Tb3.1 cell could suppress cancer cell growth in vitro and miRNA-21 can serve as a novel target candidate for human tongue cancer gene therapy.
