Establishment of human infancy hemangioma-derived endothelial cell line XPTS-1 and animal model of human infancy hemangioma
10.3760/cma.j.issn.1002-0098.2011.03.001
- VernacularTitle:婴幼儿血管瘤细胞系XPTS-1和动物模型的建立
- Author:
Peng LI
1
;
Xiao-E XIAO
;
Quan XU
;
Zheng-Tuan GUO
Author Information
1. 西安交通大学医学院第二附属医院
- Keywords:
Hemangioma;
Mode,animal;
Cell line
- From:
Chinese Journal of Stomatology
2011;46(3):129-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an immortalized human infancy hemangioma-derived endothelial cell line (HemEC) and animal model of human infancy hemangioma. Methods Hemangioma-derived endothelial cells from specimen of human infancy hemangioma were cultured in vitro and monocloed, and then its growth curve was made, karyomorphism of chromosome analyzed, morphologic characteristics observe,factor Ⅷ related antigen identified by immunohistochemical method. Vascular endothelial growth factor receptor 2(VEGFR-2) was detected by flow cytometry. HemEC were inoculated subcutaneously in athymicmouse to establish animal model of infancy hemangioma. The animal model was observed closely and its pathological characteristic was also studied. Results The cultural cells grew active, and immortalized spontaneously when they were subcultured on sixteenth generation. This cell line was cultivated for more than 70 times within one year and in good condition after freezing and resuscitating once and again, and had the morphologic character of HemEC. The cell population doubling time was 22 h. Factor Ⅷ and VEGFR-2 were expressed positively. Karyo type analysis of the cell line showed abnormal diploid with the modal chromosomal number varying between diploid and triploid. The cell line was then named XPTS-1. The animal model of infancy hemangioma was successfully established and its character of histopathology was similar with that of infancy hemangioma. Conclusions The cell line of HemEC was successfully established and immortalized spontaneously, and had the morphologic and biological character of HemEC. The animal model of infancy hemangioma was successfully established and showed the character of histopathology similar with that of infancy hemangioma.
