Visual imaging for buccal carcinoma with near-infrared fluorescent quantum dots in vivo.
- Author:
Kai YANG
1
;
Yu-an CAO
;
Zhi-gang LI
;
Xiao-qiang LÜ
;
Cheng ZHAO
;
Jia YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Squamous Cell; diagnosis; pathology; Cell Line, Tumor; Cheek; pathology; Female; Mice; Mice, Inbred BALB C; Mice, Nude; Molecular Imaging; methods; Mouth Neoplasms; diagnosis; pathology; Neoplasm Transplantation; Quantum Dots; Spectroscopy, Near-Infrared; methods; Uterine Cervical Neoplasms; pathology
- From: Chinese Journal of Stomatology 2011;46(3):134-138
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the in vivo visual imaging of buccal carcinoma with the near-infrared fluorescent quantum dots.
METHODSThe U14 cells were labeled by endocytosis with QD800 (U14/QD800) which was linked with cell-penetrating peptide. Different number of U14/QD800 was injected under the buccal mucosa of nude mice and Kunming mice separately and imaged at different time to detect the in vivo sensitivity and dynamic imaging of U14/QD800.
RESULTSThe minimum number of U14/QD800 cells which could be detected by in vivo imaging system was 1 × 10(4) in nude mice's cheek and 1 × 10(5) in Kunming mice's. The time for visual imaging of 1 × 10(4), 1 × 10(5) and 1 × 10(6) U14/QD800 cells in nude mice was 3, 7 and 16 d separately, and 3 and 10 d separately in Kunming mice.
CONCLUSIONSDue to its strong tissue penetration, near-infrared fluorescent quantum dots have great prospects in cancer early diagnosis, visual observation and individual treatment.
