Inhibitory effects of Porphyromonas endodontalis lipopolysaccharides on proliferation and differentiation of mouse osteoblast
10.3760/cma.j.issn.1002-0098.2011.03.009
- VernacularTitle:牙髓卟啉单胞菌脂多糖对小鼠成骨细胞增殖和分化的抑制作用
- Author:
Ren LI
1
;
Li-Hong QIU
;
Di YANG
;
Ming XUE
;
Ming ZHONG
Author Information
1. 中国医科大学口腔医学院
- Keywords:
Porphyromonas endodontalis;
Lipoplysacharides;
Osteoblasts;
Interleukin-6
- From:
Chinese Journal of Stomatology
2011;46(3):162-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis (Pe) on osteoblast cell proliferation and the activity of alkaline phosphatase (ALP) and interleukin (IL)-6 secretion and to investigate the role of Pe-LPS in osteoblast proliferation and differentiation. Methods MC3T3-E1 cells were treated with different concentrations of Pe-LPS ( 10, 25,50 mg/L)respectively. The relative growth rate(RGR) was detected by methyl thiazolyl tetrazolium (MTT)at different time point ( 12,24,48,72 h). MC3T3-E1 cells were also stimulated with 10,25 or 50 mg/L PeLPS for 6,12,24 and 48 h. The activity of ALP was detected by enzyme kinetics assay and the secretion of IL-6 was detected by enzyme-linked immunosorbent assay ( ELISA ). Results After the stimulation with 25 or 50 mg/L Pe-LPS for 72 h, the RGR of MC3T3-E1 cells descend to 87.46% and 71.12%. The ALP activities of MC3T3-El cells were inhibited obviously ( P ＜ 0. 05 ) after the stimulation of different concentrations( 10, 25, 50 mg/L) Pe-LPS for more than 24 hours. ELISA result showed that IL-6 increased to 32. 21 ng/L treated with the 25 mg/L Pe-LPS for 6 h, 25 mg/L Pe-LPS gradually increased the expression of IL-6 from the ELISA results. Conclusions Pe-LPS can induce the secretion of IL-6 in MC3T3-E1 and decrease the ALP activities of MC3T3-E1, the differentiation of osteoblasts was inhibited. with the long-time toxicity action of Pe-LPS, the proliferation rate of MC3T3-E1 also markedly decreased.
