Relationship between Sp3 and the transcriptional regulation of enamelin gene.
- Author:
Ping LÜ
1
;
Hua TIAN
;
Xue-jun GAO
Author Information
- Publication Type:Journal Article
- MeSH: 5' Flanking Region; genetics; Ameloblasts; cytology; Animals; Cell Line; Dental Enamel Proteins; genetics; metabolism; Electrophoretic Mobility Shift Assay; Female; Gene Expression Regulation; Genes, Reporter; Luciferases; Male; Mice; Promoter Regions, Genetic; Rats; Rats, Sprague-Dawley; Regulatory Elements, Transcriptional; Sp3 Transcription Factor; genetics; metabolism; Transcription, Genetic; Transcriptional Activation; Transfection
- From: Chinese Journal of Stomatology 2011;46(3):165-167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Sp3 in the transcriptional regulation of enamelin gene.
METHODSBy bioinformatic analysis, a putative responsive element for Sp3 was identified. Electrophoretic mobility shift assay was used to examine the interaction between Sp3 and enamelin. 5'-flanking regulatory region of enamelin was cloned and ligated into pGL3-basic luciferase vector. Sp3 and the Enam-luc were cotransfected into mouse ameloblast-like cell line, and the activity of luciferase was examined.
RESULTSThe results showed that Sp3 could not directly bind to the enamelin regulation region and activate enamelin transcription.
CONCLUSIONSSp3 might not be involved in transcriptional regulation of enamelin gene via an indirect interaction.
