Relationship between Sp3 and the transcriptional regulation of enamelin gene
10.3760/cma.j.issn.1002-0098.2011.03.010
- VernacularTitle:转录因子Sp3与釉蛋白基因转录调控关系的研究
- Author:
Ping L(U)
1
;
Hua TIAN
;
Xue-Jun GAO
Author Information
1. 北京大学口腔医学院口腔医院
- Keywords:
Sp3 transcription factor;
Dental enamel proteins;
Transcriptional regulation
- From:
Chinese Journal of Stomatology
2011;46(3):165-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Sp3 in the transcriptional regulation of enamelin gene. Methods By bioinformatic analysis, a putative responsive element for Sp3 was identified.Electrophoretic mobility shift assay was used to examine the interaction between Sp3 and enamelin.5'-flanking regulatory region of enamelin was cloned and ligated into pGL3-basic luciferase vector. Sp3 and the Enam-luc were cotransfected into mouse ameloblast-like cell line, and the activity of luciferase was examined. Results The results showed that Sp3 could not directly bind to the enamelin regulation region and activate enamelin transcription. Conclusions Sp3 might not be involved in transcriptional regulation of enamelin gene via an indirect interaction.
