Effect of rapamycin combined with cisplatin on head and neck squamous cancer cells regulated by CCL19
10.3760/cma.j.issn.1002-0098.2011.04.002
- VernacularTitle:雷帕霉素联合顺铂对CCL19调节头颈鳞状细胞癌细胞活性的影响
- Author:
Fa-Yu LIU
1
;
Zhen-Jin ZHAO
;
Peng LI
;
Xue DING
;
Chang-Fu SUN
Author Information
1. 中国医科大学口腔医学院
- Keywords:
Chemokines,CC;
Carcimona,squamous cell;
Cisplatin;
Siromus
- From:
Chinese Journal of Stomatology
2011;46(4):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine( C-C motif) ligand 19 (CCL19). Methods The role of rapamycin and cisplatin was detected on cell-cycle and apoptosis in CCL19 induced PCI-4B and PCI-37B cells by methyl thiazolyl tetrazolium (MTT) and flow cytometry(FCM). Dose-effect relationship parameters and combination index(CI) were calculated on the median-effect equation and multiple drug effect equation using computer software CalcuSyn. Statistical analysis was performed by the unpaired student's t-test.Results Rapamycin and cisplatin could respectively increase the growth arrest, the proportion of G1 phase and apoptosis of CCL19 induced cancer cells (P ＜0. 05 ). Under inhibitory concentration 50% (IC50), CI was less than 1, and in IC75, it was more than 1 in PCI-4B cells. In PCI-37B cells, under IC75, CI was less than 1, and in IC90, it was more than 1. Conclusions Rapamycin and cisplatin can inhibit CCL19-regulated PCI-4B and PCI-37B cells' survival. The two drugs have synergistic effects when used in combination.
