Expression of tumor necrosis factor in placenta tissue of pregnant rats with chronic peridontitis.
- Author:
Jing LIU
1
;
Liang-jun ZHONG
;
Fang-chuan SHI
;
Lei ZHANG
;
Qiong ZHANG
;
Da-lei SUN
Author Information
- Publication Type:Journal Article
- MeSH: Aggregatibacter actinomycetemcomitans; Animals; Animals, Newborn; Birth Weight; Chronic Periodontitis; metabolism; microbiology; Disease Models, Animal; Female; Fusobacterium nucleatum; Placenta; metabolism; Porphyromonas gingivalis; Pregnancy; Pregnancy Complications, Infectious; metabolism; microbiology; Prevotella intermedia; Random Allocation; Rats; Rats, Wistar; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Stomatology 2011;46(5):276-279
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the expression of tumor necrosis factor in placenta of pregnant rats with chronic periodontitis.
METHODSTwenty Wistar female rats were randomly divided into two groups, control (n = 8) and experimental group (n = 12). The periodontitis model was established in the experimental group. The females and males in the two groups got together four weeks later. Nineteen days after pregnancy all rats were executed and placenta collected. The delivery time and neonatal birth weight were recorded and the pathological changes of periodontal tissue observed. Tumor necrosis factor (TNF) expression was examined in placenta by real-time quantitative polymerase chain reaction analysis.
RESULTSThe animal model of chronic periodontitis was successfully established. Experimental group delivered 30 offspring and the control group 56 offspring. The average number of pups born alive per litter in experimental group (4.1 ± 2.2) was significantly lower than that in control group (9.2 ± 2.2), P < 0.05. The birth weight of pups in experimental group [(5.01 ± 0.43) g] was significantly lower than that in the control group [(5.79 ± 0.53) g], P < 0.05. The relative quantitative expression of TNF was (1.807 ± 0.265) in experimental group the and (1.003 ± 0.021) in the control group (P = 0.001).
CONCLUSIONSChronic periodontitis may be related to preterm low birth weight.
