Expression of tumor necrosis factor in placenta tissue of pregnant rats with chronic peridontitis
10.3760/cma.j.issn.1002-0098.2011.05.005
- VernacularTitle:慢性牙周炎孕鼠胎盘中肿瘤坏死因子的表达
- Author:
Jing LIU
1
;
Liang-Jun ZHONG
;
Fang-Chuan SHI
;
Lei ZHANG
;
Qiong ZHANG
;
Da-Lei SUN
Author Information
1. 新疆医科大学第五附属医院
- Keywords:
Periodontitis;
Premature birth;
Models,animal;
Tumor necrosis factors
- From:
Chinese Journal of Stomatology
2011;46(5):276-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of tumor necrosis factor in placenta of pregnant rats with chronic periodontitis.Methods Twenty Wistar female rats were randomly divided into two groups,control(n=8)and experimentalgroup(n=12).The periodontitis model was established in the experimental group.The females and males in the two groups got together four weeks later.Nineteen days after pregnancy all rats were executed and placenta collected.The delivery time and neonatal birth weight were recorded and the pathological changes of periodontal tissue observed.Tumor necrosis factor(TNF)expression was examined in placenta by real-time quantitative polymerase chain reaction analysis.Results The animal model of chronic periodontitis was successfully established.Experimental group delivered 30 offspring and the control group 56 offspring.The average number of pups born alive per liner in experimental group(4.1±2.2)Was significantly lower than that in control group(9.2±2.2),P<0.05.The birth weight of pups in experimental group[(5.01±0.43)g]wag significantly lower than that in the control group[(5.79±0.53)g],P<0.05.The relative quantitative expression of TNF was(1.807±0.265)in experimental group the and (1.003±0.021)in the control group(P=0.001).Conclusions Chronic periodonfitis may be related to preterm low birth weight.
