Effects of Bushenguchiwan on expression of matrix metalloproteinase-13 in rats' periodontium.
- Author:
Qin WEN
1
;
Heng YANG
;
Yi DING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; therapeutic use; Epithelial Cells; enzymology; Female; Fibroblasts; enzymology; Gingiva; cytology; enzymology; Male; Matrix Metalloproteinase 13; metabolism; Periodontitis; drug therapy; enzymology; microbiology; Periodontium; cytology; enzymology; Porphyromonas gingivalis; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Stomatology 2011;46(5):280-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of Bushenguchiwan on expression of matrix metalloproteinase-13 (MMP-13) in periodontium of rats with experimental periodontitis.
METHODSThe model of experimental periodontitis of rats was established and treated by Bushenguchiwan with different doses. The periodontal tissues from groups of different doses were immunohistochemically stained by antibody of MMP-13. The expression of MMP-13 was examined and semi-quantitative analysis of signals performed by integrated absorbance.
RESULTSMMP-13 was intensely positive in gingival epithelial cells and periodontal fibroblasts in periodontitis models and negative in normal rat periodontal tissues. After 30 days of Bushenguchiwan treatment with high dose, middle dose and low dose, the expression of MMP-13 (2.9103 ± 0.5534, 3.6588 ± 0.4330, 4.4550 ± 0.4255) was down-regulated respectively compared with model rats (5.3233 ± 0.7993), P < 0.05. After 60 days of treatment the expression of MMP-13 (2.1855 ± 0.5381, 2.8558 ± 0.4759, 3.8980 ± 0.5885) was down-regulated more significantly. with model rats (6.2693 ± 0.4538), P < 0.05.
CONCLUSIONSBushenguchiwan could down-regulate the expression of MMP-13 in rats' periodontium and the high dose group had better effect.
