Effects of Bushenguchiwan on expression of matrix metalloproteinase-13 in rats'periodontium
10.3760/cma.j.issn.1002-0098.2011.05.006
- VernacularTitle:补肾固齿丸对大鼠牙周组织表达基质金属蛋白酶13的影响
- Author:
Qin WEN
1
;
Heng YANG
;
Yi DING
Author Information
1. 重庆医科大学附属口腔医院
- Keywords:
Rats;
Matrix metalloproteinase 13;
Bushenguehiwan;
Experimental periodontitis
- From:
Chinese Journal of Stomatology
2011;46(5):280-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Bushenguchiwan on expression of matrix metalloproteinase-13(MMP-13)in periodontium of rats with experimental periodontitis.Methods The model of experimental periodontitis of rats was established and treated by Bushenguchiwan with different doses.The periodontal tissues from groups of different doses were immunohistochemically stained by antibody of MMP-13.The expression of MMP-13 was examined and semi-quantitative analysis of signals performed by integrated absorbance.Results MMP-13 was intensely positive in gingival epithelial cells and periodontal fibroblasts in periodontitis models and negative in normal rat periodontal tissues.After 30 days of Bushenguchiwan treatment with high dose,middle dose and low dose,the expression of MMP-13(2.9103±0.5534,3.6588±0.4330,4.4550±0.4255)was down-regulated respectively compared with model rats (5.3233±0.7993),P<0.05.After 60 days of treatment the expression of MMP-13(2.1855±0.5381,2.8558±0.4759,3.8980±0.5885)was down-regulated more significantly.with model rats(6.2693±0.4538).P<0.05.Conclusions Bushenguchiwan could down-regulate the expression of MMP-13 in rats'periodontium and the high dose group had better effect.
