Expression of periodontal ligament-associated protein-1 in normal periodontal tissues and cells in rat.
- Author:
Pan-Pan ZHANG
1
;
Shu LI
;
Pi-Shan YANG
;
Jing SUN
;
Chao HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Extracellular Matrix Proteins; genetics; metabolism; Immunohistochemistry; Male; Periodontal Ligament; cytology; metabolism; Periodontium; metabolism; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Stomatology 2011;46(6):338-341
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the expression of periodontal ligament-associated protein-1 (PLAP-1) in the periodontal tissues and periodontal ligament cells (PDLC).
METHODSThe PLAP-1 expression in normal periodontal tissue was examined by immunohistochemistry. The protein expression and mRNA transcription of PLAP-1 in PDLC were investigated by immunocytochemistry and reverse transcription-polymerase chain reaction.
RESULTSPLAP-1 was expressed in periodontium but not in cementum, alveolar bone and gingival tissues. PLAP-1 expression was observed in cell plasma, but not in nuclei. There was a 350 bp electrophoresis band representing PLAP-1 mRNA.
CONCLUSIONSPLAP-1 may play a role in physiology of periodontal tissues and cells in normal adult rats.
