Role of transcription factor special AT-rich binding protein 2 in the osteoblasts differentiation of bone marrow stromal cells.
- VernacularTitle:特殊富含AT序列结合蛋白2在诱导骨髓基质细胞成骨分化中的作用
- Author:
Qing WANG
1
;
You-Cheng YU
;
Zhang-Yu GU
;
Wei BI
;
Jian SUN
Author Information
- Publication Type:Journal Article
- MeSH: Activating Transcription Factor 4; metabolism; Animals; Bone Marrow Cells; metabolism; pathology; Cell Differentiation; Cell Movement; Cells, Cultured; Core Binding Factor Alpha 1 Subunit; metabolism; Integrin-Binding Sialoprotein; metabolism; Male; Matrix Attachment Region Binding Proteins; genetics; metabolism; Osteoblasts; cytology; Osteogenesis; Plasmids; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Stromal Cells; metabolism; pathology; Thy-1 Antigens; metabolism; Transcription Factors; genetics; metabolism; Transfection
- From: Chinese Journal of Stomatology 2011;46(6):360-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of transcription factor special AT-rich binding protein 2 (SATB2) in the osteoblasts differentiation of bone marrow stromal cells (BMSC) in vitro.
METHODSRats bone marrow stromal cells were isolated by Percoll sedimentation and the cells were placed and allowed to attach for three times. After passages, expression plasmid pBABE-hygro-satb2 was constructed, then transfected into BMSC. BMSCs were inoculated in conditioned medium and osteogenic factors were detected by western blotting and reverse transcription polymerase chain reaction (RT-PCR).
RESULTSThe morphological observation of BMSC showed either spindle or polygonal pattern. The cellular phenotypic marker of the third passage was CD29 positive and CD34 negative. The growth curve possessed "S" pattern. The intensity of calfilication in BMSC was higher in SATB2 transfection group (IA value 125974 ± 241) than that in the control groups (IA value 178486 ± 406). Moreover, cell migration rate increased in SATB2 transfection group [width of scratch (0.72 ± 0.01) mm] compared with control group [width of scratch (0.83 ± 0.03) mm]. In addition, the mRNA expression of osteogenic factors runt-related transcription factor 2, Osterix, activating transcription factor 4, integrin-binding sialoprotein were upregulated.
CONCLUSIONSCells cultured with this method have general biological characteristics and osteogenic differentiation potential in vitro. SATB2 can promote osteoblasts differentiation of BMSC.
