Mechanisms of protection effect of bcl-2 gene transfection on heat-stressed cardiomyocytes.
- Author:
Xue-li SONG
1
;
Ling-jia QIAN
;
Feng-zhi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caspase 3; metabolism; Cells, Cultured; Genes, bcl-2; Heat-Shock Response; genetics; Myocytes, Cardiac; cytology; metabolism; Proton-Translocating ATPases; metabolism; Rats; Transfection
- From: Chinese Journal of Applied Physiology 2002;18(4):347-349
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the mechanisms of protection of bcl-2 gene transfection against heat-stressed cardiomyocytes.
METHODSCardiomyocytes were isolated and cultured. bcl-2 was transfected into cardiomyocytes with Lipofectamine transfection methods. The cardiomyocytes were stressed by heat. The change of H+ -ATPase synthesis activity of cardiomyocytes mitochondria caused by bcl-2 transfection was measured by chemical radiation method. The changes of Caspase 3 activity of cardiomyocytes caused by bcl-2 transfection was measured by fluorometric analysis.
RESULTSbcl-2 transfection could increase the H+ -ATPase synthesis activity of cardiomyocytes mitochondria under heat stress at 41 degrees C and 43 degrees C and could decrease the Caspase 3 activity of cardiomyocytes under heat stress at 41 degrees C and 43 degrees C.
CONCLUSIONThe protection effect of bcl-2 transfection on heat-stressed cardiomyocytes may be associated with preserved H+ ATPase synthesis activity of cardiomyocytes mitochondria and the activity of Caspase 3 of cardiomyocytes.
