Effect of vasonatrin peptide on the expression of C-type natriuretic peptide receptor in hypoxic rat hearts.
- Author:
Jun YU
1
;
Miao-zhang ZHU
;
Bao-ying CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atrial Natriuretic Factor; blood; pharmacology; Hypoxia; metabolism; Male; Natriuretic Peptide, C-Type; metabolism; Natriuretic Peptides; metabolism; Rats; Rats, Sprague-Dawley; Receptors, Atrial Natriuretic Factor; metabolism
- From: Chinese Journal of Applied Physiology 2002;18(4):350-353
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of vasonatrin peptide (VNP) on the expression of C-type natriuretic peptide receptor (NPR-C) in hypoxic rat hearts.
METHODSRats were divided randomly into three groups: control group, hypoxia group(3-28 d) and VNP (25-75 microg/kg per day) + hypoxia group. The plasma concentration of atrial natriuretic peptide (ANP) in rats was measured by the means of radioimmunoassay. Furthermore, quantitative PCR was used to examine the NPR-C mRNA level in rat hearts.
RESULTSThe plasma concentration ANP in rats was significantly higher than that of control group, and VNP (75 microg/kg per day) made it more higher. Hypoxia for 3 day of had no significant effect on the NPR-C mRNA level in rat hearts, while hypoxia for 7-28 d significantly increased the level of NPR-C mRNA in a time dependent manner. VNP (50-75 microg/kg per day) significantly reduced the NPR-C mRNA level in rat hearts in a dose dependent manner.
CONCLUSIONVNP increases the plasma concentration of ANP in hypoxic rats. Hypoxia can increase expression of NPR-C in rat hearts significantly, which can be inhibited by VNP.
