Inhibitory effects of kappa-opioid receptor stimulation on cultured myocardial cells.
- Author:
Yan-ling YANG
1
;
Hong-xin WANG
;
Yu-hong YANG
Author Information
- Publication Type:Journal Article
- MeSH: 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; pharmacology; Animals; Cell Growth Processes; Cells, Cultured; Myocytes, Cardiac; metabolism; Rats; Rats, Sprague-Dawley; Receptors, Opioid, kappa; metabolism
- From: Chinese Journal of Applied Physiology 2002;18(4):358-361
- CountryChina
- Language:Chinese
-
Abstract:
AIMIn order to study the effects of kappa-opioid receptor activation, we used cultured cardiomyocytes to study the inhibitory effects of U50,488H on cellular proliferation, protein content in the presence or absence of nor-binaltorphimine (nor-BNI).
METHODSThe cellular proliferation was determined with crystal violet staining and the protein content was assayed with Lowry's method.
RESULTSA kappa-opioid receptor agonist U50,488H at 0.1 micromol/L-10 micromol/L inhibited the cellular proliferation and protein content of cultured myocardial cells in a dose-dependent manner. The inhibitory effects of U50,488H were completely blocked by pretreatment with nor-BNI, a specific kappa-opioid receptor antagonist at 1 micromol/L.
CONCLUSIONU50,488H inhibited the cultured myocardial cells' growth. The inhibitory effects of U50,488H are involved in mediating the action of kappa-opioid receptor stimulation.
