Measurement of nitrate and nitrite concentrations in biological fluids using high performance liquid chromatography.
- Author:
Xiu-yu CUI
1
;
Ling LI
;
Guo-wei LU
Author Information
- Publication Type:Journal Article
- MeSH: Body Fluids; chemistry; Chromatography, High Pressure Liquid; methods; Nitrates; analysis; Nitrites; analysis
- From: Chinese Journal of Applied Physiology 2002;18(4):394-396
- CountryChina
- Language:Chinese
-
Abstract:
AIM AND METHODSNew method to analyse nitrate and nitrite concentrations in saliva, serum and urine was developed using high performance liquid chromatography.
RESULTSThe whole isolation process was completed in less than 7 minutes, the determination linearity of nitrate and nitrite were 0.7 ng-100 ng and 5 ng-100 ng, respectively. MINIMUM: Detectable limits of nitrate and nitrite were 0.3 ng and 2 ng, respectively. Nitrate recovery ratio was 99%-102% and nitrite recovery ratio was 99%-104%. The RSD of nitrate and nitrite was 0.8% and 1.7%, respectively.
CONCLUSIONIn comparison with other methods available, the present method seems to be simpler, more sensitive and specific.
