Hyperglycemia inhibited taurine transport and taurine transporter gene expression in cultured rat cardiomyocytes.
- Author:
Yan-rong SHI
1
;
Lin GAO
;
Shu-heng WANG
;
Yong-zheng PANG
;
Chaoshu TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Glucose; pharmacology; Hyperglycemia; metabolism; Membrane Glycoproteins; genetics; metabolism; Membrane Transport Proteins; genetics; metabolism; Myocytes, Cardiac; drug effects; metabolism; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Taurine; metabolism
- From: Chinese Journal of Applied Physiology 2003;19(2):132-136
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the alterations of taurine transport, and taurine transporter (TAUT) mRNA by hyperglycemia in cultured rat cardiomyocytes.
METHODS3H-taurine measured the amount of taurine uptake. TAUT mRNA consents were measured using quantitative RT-PCR.
RESULTSThe cellular uptake amounts of taurine in seven groups increased with incubation time, and near to be saturated after 5 min. The uptake amount of 10, 20, and 30 mmol/L glucose groups was obviously lower than that of the control group (P < 0.05 or P < 0.01). In 30 mmmol/L glucose, taurine release obviously was decreased, as compared with that of the control. Exposure of cells to 10, 20, and 30 mmmol/L glucose decreased taurine uptake in a concentration-dependent fashion. Exposure to hyperglycemia did not affect the Km of the TAUT, but the apparent Vmax were significantly decreased (P < 0.05). In 20 and 30 mmmol/L groups, TAUT mRNA contents of myocardial cells were significantly reduced, as compared with the control group (P < 0.05).
CONCLUSIONThe data suggests that there are dysfunction of taurine uptake and downregulation of TAUT gene expression by glucose in cultured rat cardiomyocytes.
