The protective effects of ischemic preconditioning on reperfusion injury of rat lung.
- Author:
Jian-hua LI
1
;
Zhi-ping SI
;
Jia-yi LU
;
Xue-ping FAN
;
Ji-de XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ischemic Preconditioning; methods; Lung; blood supply; metabolism; pathology; Pulmonary Surfactant-Associated Proteins; secretion; Rats; Rats, Wistar; Reperfusion Injury; prevention & control
- From: Chinese Journal of Applied Physiology 2003;19(2):137-140
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of ischemic preconditioning on reperfusion injury of rat lung.
METHODSRat isolated lungs (n=8 in each group) were stabilized and perfused by Krebs-Henseleit solution on a modified langendorff perfusion apparatus. 36 wistar rats were divided into three groups: ischemic preconditioning group, ischemia/reperfusion group and control group. Mean pulmonary artery pressure, wet/dry ratio, pulmonary surfactant phospholipid and alveolar surface tension in bronchoalveolar lavage fluid and electron microscope were detected.
RESULTSThe morphological changes of lung injury were alleviated in the preconditioning group under electron microscope. Wet/dry ratio, mean pulmonary artery pressure and SA/LA ratio were significantly lower in the preconditioning group after ischemic/reperfusion (P < 0.01). Total phospholipid and large aggregate in the BALF were significantly increased in the preconditioning group (P < 0.01). Small aggregate showed no change in three groups. Surfactant activity test showed that surface tension markedly decreased in IPC group (P < 0.01).
CONCLUSIONThese results indicates that ischemic preconditioning may have a protective effect in ischemic/reperfusion injuries lung by ameliorating the content and function of surfactant phospholipid.
