Effects of fluoride on ionized calcium and calcium channel in osteoblast-like cell in vitro.
- Author:
Kun HUA
1
;
Hong ZHAO
;
Min HUANG
;
Guang-sheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Calcium; physiology; Calcium Channels; physiology; Cell Line; Fluorides; toxicity; Humans; Osteoblasts; cytology; drug effects; metabolism; Patch-Clamp Techniques
- From: Chinese Journal of Applied Physiology 2003;19(2):179-181
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of fluoride (F-) on ionized calcium ([Ca2+]i) and calcium channel in osteoblast-like cell (OBLs).
METHODSInvestigating [Ca2+]i was by fluorospectrophotometry and recording the calcium currents of OBLs was using whole patch-clamp technique.
RESULTSThe addition of fluoride to the medium made a rapid and significant increase in free [Ca2+]i, especially in 100 ng F- /ml group. There were significances between control group and 50, 100 ng F- /ml groups. And 25 ng/ml F- could stimulate OBLs calcium channel open. Compared with control group, fluoride could significantly increase the amplitudes of calcium currents (P < 0.01), furthermore the activation was in dose-dependent manner.
CONCLUSIONFluoride can make calcium channel open and then increase the concentrations of [Ca2+]i.
