Effects of genistein on proliferation and differentiation of osteoblast.
- Author:
Yue-hong ZHANG
1
;
Lan-xing GAO
;
Hong JIN
;
Zhi-qin XU
;
Wen-kao NAN
;
Xian-yuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Collagen; biosynthesis; DNA; biosynthesis; Genistein; pharmacology; Osteoblasts; cytology; drug effects; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2003;19(2):182-184
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures.
METHODSOsteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit.
RESULTSGenistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity.
CONCLUSIONGenistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.
