The effects of cell growth factors on proliferation and migration of coronary artery endothelial cells.
- Author:
Yi-Feng JIANG
1
;
Xiao-Yun LIN
;
Shuang-Hong CHEN
;
Feng ZHAO
;
Chuan-Xin LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cattle; Cell Division; drug effects; Cell Movement; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Coronary Vessels; cytology; Culture Media, Conditioned; Endothelial Cells; drug effects; Endothelium, Vascular; cytology; Hepatocyte Growth Factor; pharmacology; Vascular Endothelial Growth Factor A; pharmacology
- From: Chinese Journal of Applied Physiology 2003;19(3):227-229
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on proliferation and migration of bovine coronary artery endothelial cells (BCAEC) in vitro.
METHODSBCAECs were isolated and cultured in vitro, and divided into control group, VEGF group and HGF group. BCACEs proliferation were measured using MTT, and their migration was observed using reverse microscope.
RESULTSThe OD value of control, VEGF and HGF group were 0.22 +/- 0.01, 0.40 +/- 0.14, 0.44 +/- 0.15 respectively. The proliferation ratio of BCAECs in VEGF and HGF group was 81.8% +/- 16.9%, 100.0% +/- 21.1% respectively. There was no migration in control group, but significant migration in VEGF and HGF group.
CONCLUSIONBoth VEGF and HGF can promote proliferation and migration of BCAECs, the effect of HGF is stronger than VEGF.
