Effects of astragaloside on IGF-1 and associated protein expression in mice with acute viral myocarditis.
- Author:
Chun-Zhi HE
1
;
Shuang-Jie LI
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Animals; Coxsackievirus Infections; drug therapy; metabolism; Drugs, Chinese Herbal; therapeutic use; Enterovirus B, Human; Insulin-Like Growth Factor Binding Protein 3; analysis; Insulin-Like Growth Factor I; analysis; genetics; Male; Mice; Mice, Inbred BALB C; Myocarditis; drug therapy; metabolism; Myocardium; chemistry; RNA, Messenger; analysis; Receptor, IGF Type 1; analysis; Saponins; therapeutic use; Triterpenes; therapeutic use
- From: Chinese Journal of Contemporary Pediatrics 2011;13(9):751-754
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of astragaloside on the expression of insulin-like growth factor-1 (IGF-1) and associated proteins in mice with viral myocarditis.
METHODSSixty-five 4-week-old BALB/C mice were randomly divided into 5 groups: normal control, astragaloside control, untreated myocarditis, low-dose and high-dose astragaloside-treated myocarditis. The BALB/C mice in the later three groups were intraperitoneally injected with CVB3. The low-dose and high-dose astragaloside-treated myocarditis groups were given astragaloside of 0.07 and 0.6 mg/kg•d, respectively by intragastric administration. Fifteen days later, the samples of blood and muscular tissues were obtained. The expression of IGF-1 in plasma was measured using ELISA. The levels of IGF-1 and associated proteins in muscular tissues were measured by immunohistochemistry. The expression of IGF-1 mRNA in muscular tissues was examined by RT-polymerase chain reaction (RT-PCR).
RESULTSThe expression of IGF-1 and associated proteins increased significantly in mice infected with CVB3. High-dose astragaloside treatment reduced the expression of IGF-1 and associated proteins, but low-dose astragaloside did not.
CONCLUSIONSHigh-dose astragaloside may reduce the expression of IGF-1 and associated proteins in mice with acute viral myocarditis, possibly thus providing protective effects on muscular tissues.
