A genome sequence of novel SARS-CoV isolates: the genotype, GD-Ins29, leads to a hypothesis of viral transmission in South China.
- Author:
E'de QIN
1
;
Xionglei HE
;
Wei TIAN
;
Yong LIU
;
Wei LI
;
Jie WEN
;
Jingqiang WANG
;
Baochang FAN
;
Qingfa WU
;
Guohui CHANG
;
Wuchun CAO
;
Zuyuan XU
;
Ruifu YANG
;
Jing WANG
;
Man YU
;
Yan LI
;
Jing XU
;
Bingyin SI
;
Yongwu HU
;
Wenming PENG
;
Lin TANG
;
Tao JIANG
;
Jianping SHI
;
Jia JI
;
Yu ZHANG
;
Jia YE
;
Cui'e WANG
;
Yujun HAN
;
Jun ZHOU
;
Yajun DENG
;
Xiaoyu LI
;
Jianfei HU
;
Caiping WANG
;
Chunxia YAN
;
Qingrun ZHANG
;
Jingyue BAO
;
Guoqing LI
;
Weijun CHEN
;
Lin FANG
;
Changfeng LI
;
Meng LEI
;
Dawei LI
;
Wei TONG
;
Xiangjun TIAN
;
Jin WANG
;
Bo ZHANG
;
Haiqing ZHANG
;
Yilin ZHANG
;
Hui ZHAO
;
Xiaowei ZHANG
;
Shuangli LI
;
Xiaojie CHENG
;
Xiuqing ZHANG
;
Bin LIU
;
Changqing ZENG
;
Songgang LI
;
Xuehai TAN
;
Siqi LIU
;
Wei DONG
;
Jun WANG
;
Gane Ka-Shu WONG
;
Jun YU
;
Jian WANG
;
Qingyu ZHU
;
Huanming YANG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; Cluster Analysis; Gene Components; Genetic Variation; Genome, Viral; Genotype; Molecular Sequence Data; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; SARS Virus; genetics; Sequence Analysis, DNA; Severe Acute Respiratory Syndrome; genetics
- From: Genomics, Proteomics & Bioinformatics 2003;1(2):101-107
- CountryChina
- Language:English
- Abstract: We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27,864 (referred to the complete sequence of BJ01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream of the N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29, suggests a significant genetic event and differentiates it from the previously reported genotype, the dominant form among all sequenced SARS-CoV isolates. A 17-nt segment of this extra sequence is identical to a segment of the same size in two human mRNA sequences that may interfere with viral replication and transcription in the cytosol of the infected cells. It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development.
