Polymorphism profile of nine short tandem repeat Loci in the Han chinese.
- Author:
Shuangding LI
;
Chunxia YAN
;
Yajun DENG
;
Ruilin WANG
;
Jian WANG
;
Huanming YANG
;
Shengbin LI
- Publication Type:Journal Article
- MeSH:
Amelogenin;
China;
Dental Enamel Proteins;
genetics;
Electrophoresis;
Ethnic Groups;
genetics;
Forensic Medicine;
methods;
Gene Frequency;
Genetics, Population;
Genotype;
Heterozygote;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Sex Determination Analysis;
methods;
Tandem Repeat Sequences;
genetics
- From:
Genomics, Proteomics & Bioinformatics
2003;1(2):166-170
- CountryChina
- Language:English
-
Abstract:
Nine short tandem repeat (STR) markers (D3S1358, VWA, FGA, THO1, TPOX, CSFIPO, D5S818, D13S317, and D7S820) and a sex-identification marker (Amelogenin locus) were amplified with multiplex PCR and were genotyped with a four-color fluorescence method in samples from 174 unrelated Han individuals in North China. The allele frequencies, genotype frequencies, heterozygosity, probability of discrimination powers, probability of paternity exclusion and Hardy-Weinberg equilibrium expectations were determined. The results demonstrated that the genotypes at all these STR loci in Han population conform to Hardy-Weinberg equilibrium expectations. The combined discrimination power (DP) was 1.05 x 10(-10) within nine STR loci analyzed and the probability of paternity exclusion (EPP) was 0.9998. The results indicate that these nine STR loci and the Amelogenin locus are useful markers for human identification, paternity and maternity testing and sex determination in forensic sciences.
