Identification and expression analysis of EST-based genes in the bud of Lycoris longituba.
- Author:
Yonglan CUI
;
Xinye ZHANG
;
Yan ZHOU
;
Hong YU
;
Lin TAO
;
Lu ZHANG
;
Jian ZHOU
;
Qiang ZHUGE
;
Youming CAI
;
Minren HUANG
- Publication Type:Letter
- MeSH:
Base Composition;
Computational Biology;
Expressed Sequence Tags;
Flowering Tops;
genetics;
Gene Expression Regulation, Plant;
Genetic Variation;
Lycoris;
genetics
- From:
Genomics, Proteomics & Bioinformatics
2004;2(1):43-46
- CountryChina
- Language:English
-
Abstract:
To obtain a primary overview of gene diversity and expression pattern in Lycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were sequenced and 4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967 contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets (totally 1,066 ESTs) had homologues in GenBank and 3,621 ESTs were novel.
