The structure, expression and function prediction of DAZAP2, a down-regulated gene in multiple myeloma.
- Author:
Yiwu SHI
;
Saiqun LUO
;
Jianbin PENG
;
Chenghan HUANG
;
Daren TAN
;
Weixin HU
- Publication Type:Letter
- MeSH:
Amino Acid Sequence;
Base Sequence;
Chromosomes, Human, Pair 12;
genetics;
Chromosomes, Human, Pair 2;
genetics;
Cytoplasm;
metabolism;
DNA Primers;
DNA, Complementary;
genetics;
Down-Regulation;
Gene Components;
Humans;
Likelihood Functions;
Models, Genetic;
Molecular Sequence Data;
Multiple Myeloma;
genetics;
metabolism;
Phylogeny;
Pseudogenes;
genetics;
RNA-Binding Proteins;
genetics;
metabolism;
Sequence Alignment;
Sequence Analysis, DNA
- From:
Genomics, Proteomics & Bioinformatics
2004;2(1):47-54
- CountryChina
- Language:English
-
Abstract:
In our previous studies, DAZAP2 gene expression was down-regulated in untreated patients of multiple myeloma (MM). For better studying the structure and function of DAZAP2, a full-length cDNA was isolated from mononuclear cells of a normal human bone marrow, sequenced and deposited to Genbank (AY430097). This sequence has an identical ORF (open reading frame) as the NM_014764 from human testis and the D31767 from human cell line KG-1. Phylogenetic analysis and structure prediction reveal that DAZAP2 homologues are highly conserved throughout evolution and share a polyproline region and several potential SH2/SH3 binding sites. DAZAP2 occurs as a single-copy gene with a four-exon organization. We further noticed that the functional DAZAP2 gene is located on Chromosome 12 and its pseudogene gene is on Chromosome 2 with electronic location of human chromosome in Genbank, though no genetic abnormalities of MM have been reported on Chromosome 12. The ORF of human DAZAP2 encodes a 17-kDa protein, which is highly similar to mouse Prtb. The DAZAP2 protein is mainly localized in cytoplasm with a discrete pattern of punctuated distribution. DAZAP2 may associate with carcinogenesis of MM and participate in yet-to-be identified signaling pathways to regulate proliferation and differentiation of plasma cells.
