Testosterone's effect on tPA and PAI-1 mRNA levels of HUVEC.
- Author:
Hong JIN
1
;
Yu-Guang LI
;
Dong-Ming WANG
;
Xue-Rui TAN
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Dose-Response Relationship, Drug; Endothelial Cells; drug effects; metabolism; Endothelium, Vascular; cytology; metabolism; Humans; Male; Plasminogen Activator Inhibitor 1; biosynthesis; genetics; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Testosterone; pharmacology; Tissue Plasminogen Activator; biosynthesis; genetics; Umbilical Veins; cytology
- From: National Journal of Andrology 2005;11(8):584-586
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of testosterone with varied concentrations on the tPA and PAI-1 mRNA levels of Human umbilical vein endothelial cells (HUVEC).
METHODSHUVEC within 2 - 3 passages were cultured with testosterone (3, 30, 3 x 10(3), 3 x 10(4) nmol/L) , and the control confluent cells were cultured in the same medium without steroid for 48 hours. RT-PCR was carried out to detect tPA and PAI-1 mRNA levels.
RESULTStPA mRNA level increased, while PAI-1 mRNA levels decreased significantly, at the testosterone concentrations ranging from 3 to 3 x 10(3) nmol/L (P < 0.05). Both tPA and PAI-1 mRNA level decreased obviously of 3 x 10(4) nmol/L group.
CONCLUSIONThe results indicated that testosterone could stimulate tPA gene expression, while decreased PAI-1 mRNA level of HUVEC, which suggested that testosterone might have beneficial effects on preventing male's thrombotic diseases.
