The changes of IGF-I in testis and epididymis on a rat model with oligozoospermia/azoospermia induced by cyclophosphamide.
- Author:
Guo-qiu SHEN
1
;
Gong-cheng LU
;
Tie-jun PAN
;
Ya-jun XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Azoospermia; chemically induced; metabolism; Cyclophosphamide; toxicity; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Epididymis; metabolism; Immunohistochemistry; Insulin-Like Growth Factor I; biosynthesis; Male; Oligospermia; chemically induced; metabolism; Rats; Rats, Sprague-Dawley; Testis; metabolism
- From: National Journal of Andrology 2005;11(9):664-669
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of the levels of IGF-I in the epididymis and the expression of IGF-I in the testis of adult male rat after the administration of cyclophosphamide.
METHODSNinety-six male adult rats (8 weeks age) were divided into 6 groups. The doses given to the rats of the groups 1 to 5 were 10, 20, 40, 80 and 100 mg/(kg x d), respectively. The remaining group was served as control. All those rats were sacrificed and IGF-I were quantitatively determined by ELISA techniques 2 and 4 weeks after the administration of the drug (by gastric fudge). Immunohistochemical SP technique was used to examine expression of IGF-I in rat testis.
RESULTSThe levels of cell factors (IGF-I) in the epididymis of the rats were gradually reduced with the increasing time and dose after administration of the drug. In the mean time the expression of IGF-I in the tissues of the testis of those rats were also gradually reduced.
CONCLUSIONIn the time of oligozoospermia/azoospermia induced by the administration of cyclophosphamide, the expression levels of IGF-I in the genetic system were significantly reduced. The possible mechanism of these changes could be attributed to the lower spermatogenesis function of the testis caused by the administration of cyclophosphamide.
