Expression and significance of the matrix metalloproteinase inhibitor RECK gene in prostate cell strains.
- Author:
Zhen-yu XU
1
;
Jian-ping GAO
;
Zheng-pu ZHANG
;
Jing-ping GE
;
Chuan-liang XU
;
Ying-hao SUN
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line, Tumor; GPI-Linked Proteins; Humans; Male; Matrix Metalloproteinase 9; biosynthesis; genetics; Membrane Glycoproteins; biosynthesis; genetics; Prostatic Neoplasms; metabolism; pathology; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: National Journal of Andrology 2005;11(10):727-730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and significance of RECK gene and MMP-9 in prostate cell strains such as BPH-1, DU45, LNCaP and PC-3.
METHODSThe expression of mRNA of RECK and MMP-9 was measured by RT-PCR and RECK protein was evaluated by Western blot.
RESULTSThe mRNA level of the RECK gene in the prostate carcinoma cell strains, such as DU45, LNCaP and PC-3, was lower than that in the benign prostate hyperplasia cell line BPH-1, while MMP-9 had a higher expression. The protein level of RECK in DU-45, LNCaP and PC-3 was lower than that in the BPH-1.
CONCLUSIONThe RECK gene is supposed to be a kind of tumor suppressor gene, which may act by inhibiting the activity of MMP-9.
