Specific expression of beta-actin during spermatogenesis in rats.
- Author:
Yi-ming CHENG
1
;
Xi-quan SHI
;
He-ming YU
;
Yan-wan WU
;
Meng-chun JIA
Author Information
- Publication Type:Journal Article
- MeSH: Actins; biosynthesis; Animals; Electrophoresis, Gel, Two-Dimensional; Male; Mass Spectrometry; Rats; Rats, Sprague-Dawley; Spermatogenesis; physiology; Testis; cytology; metabolism
- From: National Journal of Andrology 2005;11(10):755-760
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the stage-specific expression proteins during rats spermatogenesis, and to investigate the beta-actin expression and localization in the tissues of rat testicular.
METHODSHighly enriched type A spermatogonia, pachytene spermatocytes and round spermatids were isolated by STAPUT method (sedimentation velocity at unit gravity, with 2% - 4% BSA gradient in DMEM/F12 medium) respectively to get the total proteins. The difference of protein expression between the three kinds of cells was analyzed by two-dimensional electrophoresis. Then the distribution of beta-actin in rat testicular tissues was investigated using specific anti-beta-actin antibodies by immunohistochemical method.
RESULTSbeta-actin was identified as a stage-specific expression protein by two-dimensional electrophoresis. beta-actin protein was more strongly expressed in type A spermatogonia and pachytene spermatocytes, but not in round spermatids. The immunohistochemical results showed that beta-actin was mainly located in the cytoplasm of type A spermatogonia and pachytene spermatocytes and in the nuclei of nearly mature spermatids.
CONCLUSIONbeta-actin protein is a stage-specific expressed protein and may play an important role in spermatogenesis.