Morphological observation of metaphase I arrested oocytes in IVF-ET cycles.
- Author:
Ning-Yuan ZHANG
1
;
Ya-Li HU
;
Hai-Xiang SUN
;
Bin WANG
;
Zhi-Peng XU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cells, Cultured; Embryo Transfer; Female; Fertilization in Vitro; Humans; Metaphase; Oocytes; ultrastructure
- From: National Journal of Andrology 2005;11(11):838-840
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe morphological characteristics and in-vitro maturation ratio of all metaphase I arrested oocytes in IVF-ET cycles.
METHODSIn two IVF-ET cycles, maturation culture in vitro was performed and we evaluated morphological characteristics of all oocytes arrested in metaphase I.
RESULTSMetaphase I arrested oocytes presented with uniform cytoplasm, compact zone and no space between oocyte and zone. Incompact association was observed between granule cell and zone without radiated structures. There was no markedly alterations in morphological characteristics after 24, 48, 72 hours of maturation culture, respectively. All oocytes were arrested in Metaphase I and no polar growed.
CONCLUSIONAll metaphase I arrested oocytes possess of unique morphological characteristics and fail to mature following current culture assay in IVF-ET cycles.
