AKAP82 expression in sperm in asthenospermia.
- Author:
Yang LI
1
;
Ji-hong LIU
;
Tao WANG
;
Zhang-qun YE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Animals; Blotting, Western; Fluorescent Antibody Technique, Indirect; Humans; Male; Oligospermia; metabolism; Protein Precursors; biosynthesis; Rabbits; Sperm Motility; Spermatozoa; metabolism
- From: National Journal of Andrology 2005;11(12):908-911
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of AKAP82 which was one of A-kinase anchor proteins in sperm in asthenospermia.
METHODSSperm from 41 patients with asthenospermia (experimental group) and 27 healthy fertile men (control group). Motility parameters were estimated with a computer-assisted semen analysis (CASA) technique. Electrophoretic and immunoblot analysis and indirect immunofluorescence of sperm were assayed in experimental group and control group respectively.
RESULTSEleven cases in experimental group showed abnormal bands or deletion of normal band in immunoblot analysis, 2 of which were also abnormal in immunofluorescence results, and while only 2 abnormal in the control. A significant difference between experimental and control group was observed (P < 0.05).
CONCLUSIONAbnormality of AKAP82 expression in sperm may be one of the etiological factors in asthenospermia.
