Cloning and expression of androgen response elements of prostate specific antigen promoter.
- Author:
Yu HU
1
;
Xue-jun SHANG
;
Jing-ping GE
;
Yi SUN
;
Yu-feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Androgens; metabolism; Cell Line, Tumor; Cloning, Molecular; Humans; Male; Promoter Regions, Genetic; Prostate; metabolism; Prostate-Specific Antigen; biosynthesis; genetics; Response Elements; Transfection
- From: National Journal of Andrology 2005;11(12):930-932
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide a possible targeted gene therapy scheme for prostate cancer, and explore the expression efficiency and tissue-specific expression of prostate specific antigen (PSA) promoter.
METHODSThree plasmids with egfp, pa-EGFP(including ARI, ARII), pba-EGFP (including ARI, ARII, ARIII) and pdeltaba-EGFP (including ARI, ARII and mutated ARIII) were designed, and the expression status was observed by transfecting into HepG2, SMMC-7721, Hela and PC-3.
RESULTSIn prostate cancer cell PC-3, pba-EGFP expressed more GFP than pa-EGFP and pdeltaba-EGFP, which showed that ARIII could notably increase the transcription efficiency of PSA promoter. Further, there was no GFP expression in HepG2, SMMC-7721 and Hela transfected with pa-EGFP, p deltaba-EGFP and pba-EGFP.
CONCLUSIONAn expression vector based on elements of the PSA gene regulatory sequences has been developed and shown to be tightly regulated in a panel of cells from tissues of various origins. With the tissue-specific functional protein, it should provide a solid platform for clinical studies.
