Expression of S518 phosphorylated Merlin and its interaction with CD44 in vestibular schwannoma
10.3321/j.issn:1673-0860.2008.12.009
- VernacularTitle:听神经瘤S518磷酸化Merlin蛋白的表达及与CD44结合特性
- Author:
Li-Hui CAI
1
;
Hao WU
;
Jing-Rong L(U)
;
Zhao-Yan WANG
Author Information
1. 上海交通大学医学院附属新华医院
- Keywords:
Neuroma,acoustic;
Neurofibromin 2;
Oxidative phosphorylation;
Antigen,CD44;
tumor suppressor proteins
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(12):910-914
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of S518 phosphorylation in Merlin on the interaction with CD44 in vestibular schwannoma and the tumor growth.Methods Thirty-five samples of vestibular schwannoma were identified by pathology.Immunohistopathology and western blot were employed to analyze the expression and localization of S518 phosphorylated Merlin in the tumor tissues.Nerve tissues that were collected during other surgical operation were used 118 control.The expression level of S518 phosphorylated Merlin was compared with clinical stages,tumor size,clinical course and cystic degeneration.Immunoprecipitation was used to evaluate the impact of S518 phosphorylation in Merlin on the interaction with CD44.Results In vestibular schwannoma,Merlin was phosphorylated at S518 and demonstrated perinuclear localization.The S518 phosphorylation level was much lower in the normal control nerve tissues than that in vestibular schwannoma tissues.There was no correlation between the phosphorylation level on Merlin and clinical stages,tumor size,clinical course and cystic degeneration.The S518 phosphorylated Merlin bound CD44 Was higher than wild-type Merlin bound CD44 in vestibular schwannoma tissues.Conclusions The affinity of Merlin to CD44 Was increased after phosphorylation at S518.Different cellular biological results might be triggered through binding to wild type Merlin and S518phosphorylated Merlin.
