Detection of hearing threshold and polymorphic molecular marker analysis of guinea pigs of mimetic aging
10.3321/j.issn:1673-0860.2008.11.008
- VernacularTitle:豚鼠老化模型听反应阈检测及基因多态性标记分析
- Author:
Min MAO
1
;
Jian-Guo ZHANG
;
Ai-Lin TAO
;
Xiao-Ling YAN
;
Shun-Lin OUYANG
;
Xu-Ping YUAN
;
Li-Zi QIU
;
Yang XIE
;
Ze-Hong ZOU
;
Jie-Zhen LU
;
Qing LI
Author Information
1. 广州医学院附属第二医院
- Keywords:
Presbycusis;
Models,animal;
Evoked potentials,auditory,brain stem;
Polymorphism,amplified fragment length
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(11):830-834
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the establishment of the mimetic aging effect in guinea pigs induced by D-galactose, and to detect the biological indicatrix associated with hearing loss and provide a new tool for molecular pathogenesis of hearing loss.Methods Total of 51 guinea pigs were randomly divided into three groups: group A ( model aging group , n = 25) , which were injected with D-galactose (200 mg ·kg-1·d-1 ) by intra peritoneum for 6 weeks, group B ( model control group , n = 18), which were given the same amount of saline only, and group C(vacant group, n = 15) were not treated.Then, The guinea pigs in group A and B were exposed in noise for 8 days, 8 hours once a day.Auditory brainstem response (ABR) was used to test the hearing threshold of guinea pigs thrice, first before the drug administered, then after 6 weeks the drug used, third after noise exposure.And colorimetry was used to analyze the activity of superoxide dismutase (SOD) and malon dialdehyde (MDA) in brain and liver tissue.The DNA of inner ear tissue was harvested and amplified fragment length polymorphism (AFLP) was used to detect the differential polymorphic markers.Results After injection, there was no significant difference in elevation of ABR threshold between the group A and group B ( t = 1.14, P>0.05 ).However, exposure of noise later, elevation in ABR threshold of ( 22.97±10.56) dB PeSPL was observed in group A, and ( 14.16±7.36) dB peSPL in group B.The was significant difference in variation of hearing threshold between group A and group B ( t = 2.78 ,P<0.05 ).The activity of SOD in brain and liver tissue in group A was lower than that in group B.the level of MDA was opposite between group A and group B.The difference between group A and group B was significant ( P<0.01 ) .A differential polymorphic marker was observed by AFLP. Conclusions The mimetic aging effect of the guinea pigs can be induced by D-galactose, and this model can not directly induce the hearing loss.The differential polymorphic marker possibly act as a predisposing factor which can greatly enhance the sensitivity of the ear to the noise.
