Evidence of bacterial biofilms in chronic rhinosinusitis
10.3321/j.issn:1673-0860.2008.11.010
- VernacularTitle:慢性鼻-鼻窦炎细菌生物膜形态观察
- Author:
Zi ZHANG
1
;
Yun-Chuan LI
;
Ye-Hua HAN
;
Wei DAI
;
Sheng-Zhong ZHANG
;
Bing ZHOU
;
Luo ZHANG
;
De-Yun WANG
;
De-Min HAN
;
Yong-Jie ZHANG
Author Information
1. 首都医科大学附属北京同仁医院
- Keywords:
Sinusitis;
Nasal mucosa;
Biofilms;
Microscopy,electron,scanning
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(11):840-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the presence of bacterial biofilms in mueosal specimens in patients operated for chronic rhinosinusitis.Methods A total of 12 subjects undergoing endoscopic sinus surgery were included.The control group was 6 patients with obstructive sleep apnea-hypopnea syndrome. Six patients with chronic rhinosinusitis were enrolled in the study group.Mucosa of uncinate process, ethmoid bulla or maxillary sinus was obtained during endoscopic sinus surgery.All the samples were prepared using standard methods for scanning electron microscopy.Patients' information such as age, gender, symptoms, sinus CT, endoscopic examination, skin prick test were recorded in detail.Results Standard-preparation scanning electron microscopy displaed denuded epithelium and disarrayed cilia in chronic rhinosinusitis patient's mucosa.In the study group, bacterial biofilms of different morphology were seen in five samples; One sample showed filamentous structure like fungi.Using strict scanning electron microscopy morphologic criteria, 83.3% samples in the study group were found to have micrographic evidence of biofilms.No bacterial biofilms were detected in the control group.Conclusions In the patients undergoing surgery for chronic rhinosinusitis, different degree of mucosal injury could be found.Bacteria biofilms of different life stages were demonstrated to be present No bacterial biofilms were detected in the control group.
