Preliminary study on biological characteristics of CD+44 stem cells in human laryngeal carcinoma
10.3321/j.issn:1673-0860.2008.11.011
- VernacularTitle:CD44+喉癌细胞的干细胞生物学特性初步研究
- Author:
Dan YU
1
;
Chun-Shun JIN
;
Yin ZHAO
;
Cheng-Bi XU
Author Information
1. 吉林大学第二医院
- Keywords:
Tumor cells,cultured;
Tumor stem cells;
Laryngeal neoplasms;
Carcinoma,squamous cell
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(11):845-850
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological characteristics of CD+44 stem cells in human laryngeal carcinoma.Methods Tumor samples were obtained from 5 patients, and then the human laryngeal carcinoma cells were cultured in vitro by primary tissue culture technique.Taking CD44 molecule as a marker to isolate CD+44 subpopulation cells from laryngeal carcinoma cells for further study.CD+44 and CD-44 cells were cultured and observed fex their development.CD+44 and CD-44 cells were compared in their functional status ( mRNA), cell cycles (GO/G1), their degree of differentiation (CK14 and Involucrin expression) and their morphologic character of the clone.Results The percentages of CD+44 cells were about49.8% -53.5% and the median was 51.3%.After culturing CD+44 cells isolated from laryngeal carcinoma could proliferate and the percentage of CD+44 remained the same.CD+44 tumor cells contained much less RNA, more GO/GI cells, expressed more CK14 protein and less lnvolucrin protein (less differentiated state).CD+44 cells were muhangular in shape with protuberances; CD-44celis showed a sharp and spindle feature.In comparison with CD-44 cells, CD+44 cells could create heterogeneous offspring by single cell culture of limiting dilution.By observing clone forming rate after single cell planting, it was found that the CD+44 cells had stronger proliferation ability.Conclusions CD+44 cells possess some characteristics of stem cells, laryngeal carcinoma stem cells maybe exist in CD+44 cells.
