Ototoxic effects of streptomycin in vestibular organotypic cultures
10.3760/cma.j.issn.1673-0860.2009.01.014
- VernacularTitle:硫酸链霉素对体外培养大鼠前庭毛细胞的损害作用
- Author:
Dong-Zhen YU
1
;
Da-Lian DING
;
Shan-Kai YIN
;
Salvi J RICHARD
Author Information
1. 上海交通大学医学院附属第六人民医院
- Keywords:
Hair cells;
vestibular;
Organ culture techniques;
Streptomycin;
Apoptosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2009;44(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ototoxic effects of streptomycin in vestibular organotypic cultures. Methods F344 rats with age at postnatal day three or four were used for this study. The maculae of saccule and utricle were routinely dissected out and cultured with serum-free medium containing various dose of streptomycin for 24 hours. The ciliary turf of vestibular hair ceils was stained with fluorescent phalloidin, and its nucleus was stained with topro-3 DNA probe. The vestibular hair cells were quantitatively counted and photographed under confocal fluorescent microscope. Results Morphological feature of vestibular hair cells were good in normal control cultures. However, the density of hair cells was decreased in evidence with increase of streptomycin sulfate concentrations. wenty-four hours after streptomycin cultures, 0.25 mmol/L streptomycin caused a 10% hair cell missing, 50% hair cell loss from 1 mmol/L streptomycin treatment, and more than 75% hair cells gone post-3 mmol/L streptomycin cultures. After streptomycin treatment, the nuclear shrinkage and fragmentation were found in vestibular hair cells, whereas the vestibular supporting cells were normal. Conclusion Streptomycin induced-vestibular hair cells lesion was in a dose dependent manner with nuclear shrinkage and fragmentation. This may suggest that streptomycin leads vestibular hair cell die through apoptosis.
