Expression and clinical significance of hypoxia-inducible factor-1α and cyclooxygenase-2 in laryngealsquamous cell carcinoma
10.3760/cma.j.issn.1673-0860.2009.01.015
- VernacularTitle:缺氧诱导因子1α和环氧化酶2在喉鳞癌组织中的表达及意义
- Author:
Cheng-Zhi XU
1
;
Pin DONG
;
Xiao-Yan LI
;
Zhi-Jie ZHANG
Author Information
1. 上海交通大学医学院附属第九人民医院
- Keywords:
Laryngeal neoplasms;
Carcinoma,squamous cell;
Hypoxia-inducible factor 1;
alpha subunit;
Cyclooxygenase 2;
Survival analysis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2009;44(1):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate tbe expression of hypoxia-inducible factor-1 alpha (HIF-1α) and cyclooxygenase-2 (COX-2) in laryngeal squamous cell carcinoma (LSCC), while determine their relationship with clinicopathologic characteristics and prognosis. Methods Tumor tissues were obtained from 60 patients who underwent resection of laryngeal carcinoma in Affiliated First People's Hospital of Shanghai Jiaotong University. Immunohistochemistry (Envision DAKO) was used to detect the expressions of HIF-1α and COX-2 in the tumor tissues. As control group, 15 cases of atypical hyperplasia, 10 cases of leukoplakia of vocal cord and 10 cases of polyp of vocal cord were studied. All patients were regularly followed up and the clinical data were collected systematically. Results Positive staining rates of HIF-1α and COX-2 were 95.0% (57/60) and 98.3% (59/60), respectively in all 60 specimen of LSCC. The positive expressions in LSCC were significantly higher than those in atypical hyperplasia, leukoplakia and polyp of vocal cord(Fisher's exact test,P<0.01). The expression of HIF-1α was correlated with COX-2 in LSCC(r=0.526, P<0.01). High level expressions of HIF-1α and COX-2 were 35.0% (21/60) and 38.3% (23/60) respectively. High level expression of HIF-1α was significantly correlated with clinical stages(X2=4.331, P<0.05) and lymph nodes metastases(Fisher's exact test, P<0.05). High level expression of COX-2 was significantly correlated with clinical stage (X2=8.539, P<0.01) and T stages (X2=6.792, P<0.01). With univariate analysis, high level expressions of HIF-1α and COX-2 were significantly associated with a worse overall survival (X2=6.003, P<0.05 and X2=9.489, P<0.01, respectively) and disease-free survival(X2=5.010,P<0.05 and X2=6.102,P<0.05, respectively). With multivariate analysis, recurrence and high level expression of COX-2 were two unfavorable prognostic factors (RR=7.104, P=0.003; RR=5.714, P=0.008). Conclusions The expressions of HIF-1α and COX-2 played an important role in the process of tumorigenesis and development of LSCC, The expression of HIF-1α was correlated with COX-2 in LSCC. COX-2 and recurrence were probably significant risk factors for prognosis of LSCC.
