Reversal effect of resveratrol on chemotherapy resistance in KBv200 cell line and underlyingmechanisms
10.3760/cma.j.issn.1673-0860.2009.01.016
- VernacularTitle:白藜芦醇对KBv200细胞多药耐药逆转作用的研究
- Author:
Fang QUAN
1
;
Cheng-En PAN
;
Shao-Qiang ZHANG
;
Li-Ying YAN
;
Liang YU
Author Information
1. 西安交通大学医学院附属第一医院
- Keywords:
Drug resistance;
multiple;
KB cells;
Apoptosis;
Stilbenes
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2009;44(1):63-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the reversal effect and potential mechanism of resveratrel on multidrug resistance of human oral epidermoid carcinoma KBv200 cells. Methods MTT assay was used to investigate reversal index of resveratrol to vincristine, adriamycin and paclitaxel. Cell apoptosis were measured by flow cytometry. RT-PCR and Western blot were used to detect mRNA and protein expression of multidrug resistant 1 (MDR1) and B cell lymphoma leukemia-2(Bcl-2). Results Resveratrol produced a synergistic effect with chemotherapeutics and obviously reversed the muhidrug resistant phenotype of KBv200 cells. The reversal fold (RF) of 200 umol/L resveratrol to vincristine, paclitaxel and adriamycin were 77.1,61.3 and 5.9, respectively. The gene array results showed that resveratrol greatly downregulated expression levels of Bcl-2 and MDR1. After treated with 100 umol/L, 200 umol/L resveratrol, the expression level of Bcl-2 and MDR1 in KBv200 cells were markedly decreased in comparison with those untreated (t were 2.98,3.51 and 3.12,4. 56,P<0.05). Conclusions Resveratrol can efficiently reverse multidrug resistance in KBv200 cells. The potential mechanism may be via inhibiting the multidrug resistant gene expressions and/or promoting cell apoptosis.
