In vitro study of directional inducible differentiation of inner ear hair cells from human adipose-derived mesenchymal stem cells.
- Author:
Xian-dao YUAN
1
;
Xi YAN
;
Hua YANG
;
Zhi-qiang GAO
;
Kang-hua LI
;
Qin HAN
;
Shan LU
;
Xiao-wei CHEN
;
Hong JIANG
;
Chun-hua ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; cytology; Animals; Cell Culture Techniques; methods; Cell Differentiation; Cells, Cultured; Chick Embryo; Coculture Techniques; Hair Cells, Auditory, Inner; cytology; Humans; Mesenchymal Stromal Cells; cytology
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(4):323-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells.
METHODSMesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro. hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers.
RESULTSMorphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells.
CONCLUSIONShAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.
