In vitro study of directional inducible differentiation of inner ear hair cells from human adipose-derived mesenchymal stem cells

VernacularTitle:人脂肪源性间充质干细胞向内耳毛细胞定向诱导分化的实验研究
Author: Xian-Dao YUAN ¹ ; Xi YAN ; Hua YANG ; Zhi-Qiang GAO ; Kang-Hua LI ; Qin HAN ; Shan LU ; Xiao-Wei CHEN ; Hong JIANG ; Chun-Hua ZHAO
Author Information

1. 中国医学科学院北京协和医学院北京协和医院
Keywords: Mesenchymal stem cells; Ear,inner; Hair cells; Cell differentiation
From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(4):323-328
CountryChina
Language:Chinese
Abstract: Objective To explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells. Methods Mesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro, hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers. Results Morphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells. Conclusions hAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.