Activation of nuclear factor-kappaB and aberrant gene expression of interleukin 6 in middle ear cholesteatoma.
- Author:
Yu XU
1
;
Ze-zhang TAO
;
Qing-quan HUA
;
Xin-chun WANG
;
Bo-kui XIAO
;
Shi-ming CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cholesteatoma, Middle Ear; genetics; metabolism; Gene Expression Regulation; Humans; Interleukin-6; genetics; metabolism; NF-kappa B; metabolism; RNA, Messenger; genetics
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(3):192-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the activation of NF-kappaB in middle ear cholesteatoma, discuss the relationship of NF-kappaB and the gene expression of IL-6 and explore the pathogenesis of middle ear cholesteatoma.
METHODSTen cases of cholesteatoma and 6 cases of normal external meatal skin were obtained from middle ear surgery. The NF-kappaB DNA binding activity and the mRNA level of IL-6 in these two kinds of tissues were detected by electrophoretic motility shift assay (EMSA) and Reverse transcription polymerase chain reaction (RT-PCR) respectively. The relationship of NF-kappaB DNA binding activity and the mRNA level of IL-6 were analyzed.
RESULTSThe NF-kappaB DNA binding activities of cholesteatoma [(15.9 +/- 8.2)%] were higher than those in normal skin [(1.36 +/- 0.94)%, t = 3.502, P < 0.05]. The expression of IL-6 mRNA was increased significantly in patients with cholesteatoma, as compared with that in the control specimens (t = 2.166, P < 0.05) and had a significant positive correlation with NF-kappaB DNA binding activity (r = 0.752, P < 0.05).
CONCLUSIONSThe IL-6 mRNA expression in cholesteatoma is closely related with the activity of NF-kappaB. It is tempting to speculate that NF-kappaB play a key role in the activation of cytokine in cholesteatoma.
